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Phospho-Troponin I (Cardiac) (Ser23/24) Antibody

Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4001

Inquiry Info. # 4001

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	28
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Troponin I (Cardiac) (Ser23/24) Antibody detects endogenous levels of cardiac troponin I only when phosphorylated on serine 23 and 24.This antibody does not cross-react with phosphorylated skeletal muscle troponin I.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-Ser23/24 peptide corresponding to residues surrounding Serine 23/24 of mouse troponin I (cardiac). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca²⁺ concentration. Troponin consists of three subunits: the Ca²⁺-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca²⁺ sensitivity by reducing the Ca²⁺ binding affinity of TnC (1-3).

Alternate Names

Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; TNNC1; TNNI3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I3, cardiac type

Database Links

UniProt ID: P19429

Entrez-Gene Id: 7137

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For Research Use Only. Not for Use in Diagnostic Procedures.

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