Render Target: STATIC
Render Timestamp: 2024-12-10T11:16:00.654Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:57:06.076
Product last modified at: 2024-10-21T18:15:08.312Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416

Filter:
  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:400
    Peptide ELISA (DELFIA) 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence.
    2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides.

    Background

    Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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