Phospho-UPF1 (Ser1107) Antibody #84283
- WB
- IP
Inquiry Info. # 84283
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Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
SMG-1 can phosphorylate UPF1 on Ser1107, creating a docking site for SMG-5 and SMG-7. This binding enables NMD and the subsequent dissociation of UPF1 from mRNA, allowing for the recycling of ribosomes, release factors, and NMD factors (6,7).
- Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
- Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
- Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
- Isken, O. et al. (2008) Cell 133, 314-27.
- Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.
- Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
- Okada-Katsuhata, Y. et al. (2012) Nucleic Acids Res 40, 1251-66.
Alternate Names
ATP-dependent helicase RENT1; delta helicase; FLJ43809; FLJ46894; hUpf1; KIAA0221; Nonsense mRNA reducing factor 1; NORF1; pNORF1; Regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; UP Frameshift 1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1; UPF1 regulator of nonsense transcripts homolog; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1 RNA helicase and ATPase; yeast Upf1p homolog
Limited Uses
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