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  3. Phospho-YAP/TAZ Antibody Sampler Kit

Phospho-YAP/TAZ Antibody Sampler Kit #52420

Inquiry Info. # 52420

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    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Phospho-YAP/TAZ Antibody Sampler Kit uses phospho-specific and control antibodies to provide an economical means of detecting the phosphorylation of YAP and TAZ proteins at critical residues that are reported to regulate YAP and TAZ protein stability. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

    Background

    YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. YAP and TAZ are structurally and functionally similar, but exhibit differential patterns of expression among cells and tissues that suggest partially non-redundant functions (1). YAP and TAZ are dynamically regulated in response to internal and external cellular signals. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with transcription factors (e.g., TEA domain proteins) that regulate the transcription of genes that control proliferation and cell survival (2). The subcellular localization of YAP and TAZ is dynamically regulated by a kinase cascade that regulates the phosphorylation status of key residues within YAP and TAZ. Phosphorylation of YAP and TAZ (e.g., Ser109, Ser127, Ser397 in YAP; Ser89 in TAZ) results in their cytoplasmic translocation, sequestration by 14-3-3 proteins, and recruitment of the β-TrCP (SCF) ubiquitin ligase complex (3,4). This complex ubiquitinates YAP and TAZ, triggering their proteolytic degradation in the proteasome, thereby altering the transcription of genes that control proliferation and cell survival (3-5).

    Alternate Names

    Hippo

    Database Links

    UniProt ID: Q9GZV5 , P46937


    Entrez-Gene Id: 25937 , 10413


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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