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9779
Pim Kinase Antibody Sampler Kit

Pim Kinase Antibody Sampler Kit #9779

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using Pim-1 (C93F2) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from K-562, Raji and KARPAS-620 cell lines using Pim-2 (D1D2) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower).

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb.

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 7

Western blot analysis of recombinant Pim-1, -2, and -3 using Pim-1 (C93F2) Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of recombinant Pim-1, Pim-2 and Pim-3 kinases using Pim-2 (D1D2) Rabbit mAb.

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Western Blotting Image 9

Western blot analysis of recombinant Pim-1, 2, and 3 using Pim-3 (D17C9) Rabbit mAb.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Bad (Ser 112) (40A9) Rabbit mAb.

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Western Blotting Image 11

Western blot analysis of extracts from COS-7 cells, untransfected () or transfected with a mouse Pim-3 construct (+), using Pim-3 (D17C9) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Bad (Ser 112) (40A9) Rabbit Monoclonal Antibody preincubated with control peptide (left) or Phospho-Bad (Ser 112) Blocking Peptide (IHC Specific) #1026 (right).

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

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Flow Cytometry Image 15

Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Pim-1 (C93F2) Rabbit mAb 3247 20 µl
  • WB
H M 34 Rabbit IgG
Pim-2 (D1D2) Rabbit mAb 4730 20 µl
  • WB
  • IP
H 40, 38, 34 Rabbit 
Pim-3 (D17C9) Rabbit mAb 4165 20 µl
  • WB
H M R 35 Rabbit IgG
Phospho-Bad (Ser112) (40A9) Rabbit mAb 5284 20 µl
  • WB
  • IHC
  • F
H M R Mk 23 Rabbit IgG
Bad (D24A9) Rabbit mAb 9239 20 µl
  • WB
H M R Mk 23 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Pim Kinase Antibody Sampler Kit provides an economical means to detect all three Pim kinases along with Bad and Phospho-Bad (Ser112). The kit contains enough primary and secondary antibody to perform two western blot experiments.

The Pim-1 (C93F2) Rabbit mAb, Pim-2 (D1D2) Rabbit mAb, and Pim-3 (D17C9) Rabbit mAb detect endogenous levels of the respective proteins. The Pim antibodies do not crossreact with other Pim family members. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. It does not detect Bad phosphorylated at other sites, nor does it detect related family members. Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val160 of human Pim-1, Cys266 of human Pim-2, Ser275 of human Pim-3, Ser112 of mouse Bad, and Pro102 of human Bad, respectively.

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).

The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).

  1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
  2. Selten, G. et al. (1986) Cell 46, 603-11.
  3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
  4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
  5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
  6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
  7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
  8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
  9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
  10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
  11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
  12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
  13. Saris, C.J. et al. (1991) EMBO J 10, 655-64.
Entrez-Gene Id
572 , 5292 , 11040 , 415116
Swiss-Prot Acc.
Q92934 , P11309 , Q9P1W9 , Q86V86
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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To Purchase # 9779T

Product Number Size Price
9779T 1 Kit (5 x 20 µl) $372.00.0
Quantity Subtotal
$0.00