Render Target: STATIC
Render Timestamp: 2024-12-12T11:45:08.361Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:53:09.536
Product last modified at: 2024-09-30T08:02:21.436Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

PINCH (5G7) Mouse mAb #11890

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PINCH (5G7) Mouse mAb recognizes endogenous levels of total PINCH protein. The antibody recognizes a protein of unknown origin at 80 kDa in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the the human PINCH protein.

    Background

    The extracellular matrix (ECM) is a complex structure of secreted macromolecules surrounding mammalian organs and tissues. Controlled interactions between cells and the ECM are important in proliferation, migration, survival, polarity, and differentiation. Cells contact the ECM primarily through focal adhesion complexes, which contain integrins, as well as multiple adaptor and signaling proteins (1). The ILK/PINCH/Parvin (IPP) adaptor complex acts at the interface of the integrin/actin connection to regulate formation of focal adhesions and integrin signaling. Roles for IPP proteins outside of the IPP complex have been proposed, including regulation of gene expression (2,3).
    PINCH, also known as LIMS1, has been shown to function as a specific regulator of gene expression in glomerular podocytes in response to TGF-β1 (4). Researchers have shown that PINCH is highly expressed in some human tumors, and that PINCH can promote resistance to ionizing radiation through activation of Akt (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.