Render Target: STATIC
Render Timestamp: 2025-03-19T10:01:50.467Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:32.610
Product last modified at: 2025-03-06T09:00:35.948Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

POFUT1 (E8F4R) Rabbit mAb (IHC Formulated) #42306

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  • IHC

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous (IHC-P)
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    POFUT1 (E8F4R) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total POFUT1 protein. Non-specific staining was observed in some neuronal tissue.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POFUT1 protein.

    Background

    Notch signaling is essential for lymphocyte development and myelopoiesis (1,2,4,6). Notch receptors are modified by O-fucosylation catalyzed by the enzyme protein O-fucosyltransferase 1 (POFUT1) (1,2,4). POFUT1 fucosylates the epidermal growth factor (EGF)-like domains found in cell surface and secreted glycoproteins, including Notch and its ligands. POFUT1 catalyzes the reaction by attaching fucose via O-glycosidic linkage to conserved serine or threonine residues in EGF-like domains (2,7). This particular modification of Notch is critical for development and cell fate; POFUT1 deficiency leads to aberrant signaling and human disease (3,5,8,9). In addition, mouse embryonic stem cells lacking POFUT1 have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or display proper Notch signaling (4,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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