PRK2 (D10A10) Rabbit mAb #8697
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H Mk B Pg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- B-Bovine
- Pg-Pig
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
PRK2 (D10A10) Rabbit mAb recognizes endogenous levels of total PRK2 protein. This antibody does not cross-react with PRK1.
Species Reactivity:
Human, Monkey, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu633 of human PRK2 protein.
Background
The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).
- Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
- Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
- Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
- Watanabe, G. et al. (1996) Science 271, 645-648.
- Amano, M. et al. (1996) Science 271, 648-650.
- Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
- Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
- Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
- Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
- Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.
Limited Uses
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