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Product last modified at: 2024-08-27T19:45:07.690Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

PRMT Antibody Sampler Kit #54513

    Product Information

    Product Description

    The PRMT Antibody Sampler Kit provides an economical means of detecting arginine N-methyltransferase (PRMT) family members using antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the PRMT Antibody Sampler Kit detects endogenous levels of its target protein.

    In western blot analysis, PRMT5 (D5P2T) Rabbit mAb also detects a 25 kDa protein of unknown identity.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu337 of human PRMT1 protein, Gly14 of human PRMT2 protein, Gly44 of human PRMT3 protein, Ala62 of human PRMT6 protein, Val233 of human PRMT7 protein, Gly323 of human PRMT8 protein, and corresponding to the amino
    terminus of human PRMT4/CARM1 protein. Monoclonal antibody is produced by immunizing animals with recombinant human PRMT5 protein.

    Background

    Arginine methylation is a prevalent PTM found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,NG-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-methylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, 6, and 8) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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