PRMT Antibody Sampler Kit #54513
Product Information
Kit Usage Information
Protocols
- 3379: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 7074: Western Blotting
- 14641: Western Blotting, Immunoprecipitation (Agarose)
- 14762: Western Blotting
- 67007: Western Blotting, Immunoprecipitation (Agarose)
- 72593: Western Blotting
- 79780: Western Blotting
- 79998: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
- 83283: Western Blotting
Product Description
Specificity / Sensitivity
Each antibody in the PRMT Antibody Sampler Kit detects endogenous levels of its target protein.
In western blot analysis, PRMT5 (D5P2T) Rabbit mAb also detects a 25 kDa protein of unknown identity.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu337 of human PRMT1 protein, Gly14 of human PRMT2 protein, Gly44 of human PRMT3 protein, Ala62 of human PRMT6 protein, Val233 of human PRMT7 protein, Gly323 of human PRMT8 protein, and corresponding to the amino
terminus of human PRMT4/CARM1 protein. Monoclonal antibody is produced by immunizing animals with recombinant human PRMT5 protein.
Background
Arginine methylation is a prevalent PTM found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,NG-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-methylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, 6, and 8) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6).
- Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72.
- Pahlich, S. et al. (2006) Biochim Biophys Acta 1764, 1890-903.
- Bedford, M.T. and Clarke, S.G. (2009) Mol Cell 33, 1-13.
- McBride, A.E. and Silver, P.A. (2001) Cell 106, 5-8.
- Gary, J.D. and Clarke, S. (1998) Prog Nucleic Acid Res Mol Biol 61, 65-131.
- Cheng, D. et al. (2007) Mol Cell 25, 71-83.
Limited Uses
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