Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb (BSA and Azide Free) #92728
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total prostate specific membrane antigen. An SDS resistant dimer at approximately 200 kDa can be detected depending on sample preparation conditions.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of human prostate specific membrane antigen protein.
Background
Prostate specific membrane antigen (PSMA, also known as FOLH1), a type II transmembrane protein of the M28 family, has both folate hydrolase and N-acetylated-alpha-linked acidic dipeptidase activity. PSMA was originally identified in the LNCaP cell line, which was derived from a prostate adenocarcinoma lymph node metastasis (1,2). PSMA is an established prostate cancer marker (3); however, it is expressed in other tissues, including kidney, liver, and urinary bladder (4), and it is associated with tumor neovasculature (5) as well. Research studies suggest that PSMA is both a potential diagnostic readout and therapeutic target (6-8).
- Horoszewicz, J.S. et al. (1987) Anticancer Res 7, 927-35.
- Israeli, R.S. et al. (1993) Cancer Res 53, 227-30.
- Ben Jemaa, A. et al. (2010) J Exp Clin Cancer Res 29, 171.
- Kinoshita, Y. et al. (2006) World J Surg 30, 628-36.
- Chang, S.S. et al. (1999) Mol Urol 3, 313-320.
- Mease, R.C. et al. (2013) Curr Top Med Chem 13, 951-62.
- Frigerio, B. et al. (2013) Eur J Cancer 49, 2223-32.
- Denmeade, S.R. et al. (2012) Sci Transl Med 4, 140ra86.
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