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51469
PhosphoPlus® PSD95 (Ser295) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® PSD95 (Ser295) Antibody Duet #51469

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PhosphoPlus® PSD95 (Ser295) Antibody Duet: Image 1
Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.
PhosphoPlus® PSD95 (Ser295) Antibody Duet: Image 2
Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb (upper) or PSD95 (D74D3) XP® Rabbit mAb #3409 (lower).
PhosphoPlus® PSD95 (Ser295) Antibody Duet: Image 3
Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
To Purchase # 51469
Cat. # Size Qty. Price
51469S
1 Kit $ 552

Kit Usage Information

Protocols

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
MW (kDa) 95

Background

Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience-dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).
JNK1 phosphorylates PSD95 at Ser295, enhancing synaptic accumulation of PSD95 and potentiating excitatory post-synaptic currents through PSD95's increased ability to recruit AMPA receptors. In addition, synaptic depression requires dephosphorylation of Ser295 (6).
  1. Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
  2. Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
  3. Cai, C. et al. (2006) J. Biol. Chem. 281, 4267-73.
  4. Yao, W.D. et al. (2004) Neuron 41, 625-38.
  5. Cline, H. (2005) Curr. Biol. 15, R203-5.
  6. Kim, M.J. et al. (2007) Neuron 56, 488-502.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
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