PSPH (E1D2X) Rabbit mAb #58258

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	25
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PSPH (E1D2X) Rabbit mAb recognizes endogenous levels of total PSPH protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly141 of human PSPH protein.

Background

Serine biosynthesis involves sequential reactions catalyzed by several metabolic enzymes, including phosphoserine phosphatase (PSPH). PSPH catalyzes the dephosphorylation of phospho-L-serine to L-serine, the last irreversible step in serine biosynthesis (1-3). Studies show that PSPH is overexpressed in melanoma samples, and this overexpression is essential for tumor growth and metastasis. PSPH knockdown in melanoma cells leads to increased 2-hydroxyglutarate (2-HG) levels. 2-HG suppresses melanoma cell growth (3).

Database Links

UniProt ID: P78330

Entrez-Gene Id: 5723

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For Research Use Only. Not For Use In Diagnostic Procedures.

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