Render Target: STATIC
Render Timestamp:
3/26/2025, 10:37:55 AM EDT
3/26/2025, 2:37:55 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:19:08.471
Product last modified at: 2025-01-07T08:01:08.646Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PTPRJ/DEP-1 (F4G8K) Rabbit mAb #28386

Filter:
  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 240
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400
    Flow Cytometry (Live) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PTPRJ/DEP-1 (F4G8K) Rabbit mAb recognizes endogenous levels of total PTPRJ/DEP-1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a fragment of the extracellular domain of human PTPRJ/DEP-1 protein.

    Background

    Protein-tyrosine phosphatase receptor type J (PTPRJ), also known as density-enhanced phosphatase-1 (DEP-1), is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ/DEP-1 functions as a tumor suppressor, and its expression is lost in multiple types of human cancers (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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