Render Target: STATIC
Render Timestamp: 2024-12-03T10:54:52.509Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-11-19 23:03:10.149
Product last modified at: 2024-11-23T09:00:12.816Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

R-Ras2 (F6T8H) Rabbit mAb #57299

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    R-Ras2 (F6T8H) Rabbit mAb recognizes endogenous levels of total R-Ras2 protein. This antibody does not cross-react with either R-Ras1 or R-Ras3 proteins.

    Species Reactivity:

    Human, Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human R-Ras2 protein.

    Background

    R-Ras2 (TC21) belongs to the R-Ras subfamily of small GTPases, which includes R-Ras1 and R-Ras3 (M-Ras). Similar to the classical Ras proteins, R-Ras2 is palmitoylated and farnesylated at its carboxy terminus, which facilitates association with membranes and potent activation of the ERK/MAPK and PI3K signaling cascades. Research studies have identified a role for R-Ras2 in the homeostasis of the adaptive immune system by demonstrating that loss of R-Ras2 expression impairs the proliferation and survival of lymphocytes in both the T cell and B cell compartments (1-3). R-Ras2 has also been implicated in human cancers. Indeed, overexpression of R-Ras2 has been observed in both lymphoid malignancies and solid tumor settings, concomitant with hyperactivation of signaling nodes that drive cell proliferation and survival (1,3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.