Render Target: STATIC
Render Timestamp: 2024-12-06T11:56:55.892Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:56:30.349
Product last modified at: 2024-11-15T12:30:10.668Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rab17 (D2B2) Rabbit mAb #8013

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 23
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab17 (D2B2) Rabbit mAb detects endogeneous levels of total Rab17 protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe173 of human Rab17 protein.

    Background

    Rab17 belongs to the Rab family of small Ras-like GTPases. It is specifically expressed in epithelial cells and is upregulated during cell polarization (1). Immunofluorescence staining studies indicate that Rab17 is associated with the perinuclear recycling endosome in nonpolarized epithelial cells and with the apical recycling endosome in polarized epithelial cells (2). The function of Rab17 remains unclear. Reports of Rab17 colocalization with internalized IgA in the apical endosome suggest that it may regulate receptor-mediated transcytosis (3). Rab17 has also been shown to regulate melanocytic filopodia formation and melanosome trafficking. siRNA knockdown of Rab17 in melanoma cells induces melanosome accumulation in the cell periphery (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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