Render Target: STATIC
Render Timestamp: 2024-10-11T10:08:41.530Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:27.964
Product last modified at: 2024-09-30T08:02:01.471Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rab29 (E4P6M) Rabbit mAb #18759

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 23
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab29 (E4P6M) Rabbit mAb recognizes endogenous levels of total Rab29 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Rab29 protein.

    Background

    Rab29 (Rab7L1) is a member of the Ras GTPase superfamily of small monomeric G proteins responsible for regulation of intracellular membrane trafficking (1,2). Rab29 is involved in maintaining the integrity of the trans-Golgi network (TGN) and has been shown to play a role in the retrograde trafficking of mannose-6-phosphate receptors (M6PR) to the TGN (3). Rab29 is a substrate of LRRK2, a kinase associated with Parkinson’s disease (PD), and is one of five genes encoded within the PD-linked PARK16 locus (4,5). It has been shown that Rab29 recruits LRRK2 to the TGN and stimulates its kinase activity. Mimicking LRRK2 phosphorylation of Rab29 at Thr71 and Ser72 appears to suppress activation of LRRK2. Thus, Rab29 acts as a regulator of LRRK2, controlling its activation and localization (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.