Render Target: STATIC
Render Timestamp: 2024-11-01T10:35:17.246Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:54:22.425
Product last modified at: 2024-10-25T21:00:09.668Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rad54 (D4W3Z) Rabbit mAb #15016

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 84
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rad54 (D4W3Z) Rabbit mAb recognizes endogenous levels of total Rad54 protein. Based on sequence, this antibody is not expected to recognize Rad54B. This antibody also cross-reacts with an unidentified protein of 200 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly246 of human Rad54 protein.

    Background

    DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Defects in both pathways have been associated with human disease, including cancer (1). The DNA repair and recombination protein RAD54-like (Rad54, RAD54L) is a Swi2/Snf2 family DNA helicase that is involved in homologous recombination DNA repair. Rad54 is a double-stranded DNA-dependent ATPase that translocates in a processive manner along double-stranded DNA. The Rad54 helicase interacts with the Rad51 recombinase to regulate its DNA binding and strand exchange activities during homologous recombination (2-4). Mutations in the corresponding RAD54L gene are associated with multiple forms of human cancer, including non-Hodgkin's lymphoma, breast cancer, and parathyroid adenoma (5-8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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