Render Target: STATIC
Render Timestamp: 2025-03-19T11:13:25.562Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:19:31.142
Product last modified at: 2025-01-01T09:02:43.373Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Raet1 (F4V7S) Rabbit mAb #77657

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 40-60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Raet1 (F4V7S) Rabbit mAb recognizes endogenous levels of total Raet1 protein. A non-specific band at 100 kDa can be observed in some cell lines.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His33 of mouse Raet1 protein.

    Background

    The activating receptor natural killer group 2, member D (NKG2D) is involved in both innate and adaptive immune response, and functions as a switch determining the activation status of natural killer (NK) cells (1,2). NKG2D binds to a diverse family of ligand molecules, which are expressed at low levels in most cells but can be upregulated by cellular stress response (1-3). Retinoic acid early transcript 1 (Raet1) is a GPI-linked cell surface protein that functions as a ligand for NKG2D, and has been shown to activate NK and T cells (3-5). In addition, Raet1 promotes tumor immune surveillance by inducing proliferation of anti-tumor cytotoxic T lymphocytes (3). Raet1 has been shown to be regulated by the STING/cGAS pathway (5), is involved in response to neuronal damage and encephalitis (6,8), and has been implicated in chronic inflammation and autoimmune disease (7,8). The NKG2D-Raet1 interaction is considered a promising target for immune-based therapies due to the specific expression of stress-induced ligands exclusively on tumor cells and strongly induced NK cell activation (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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