|Phospho-A-Raf (Ser299) Antibody 4431||20 µl||
||H M R||68||Rabbit|
|A-Raf Antibody 4432||20 µl||
||H M R||68||Rabbit|
|Phospho-c-Raf (Ser338) (56A6) Rabbit mAb 9427||20 µl||
||H M R Mk||74||Rabbit IgG|
|Phospho-c-Raf (Ser289/296/301) Antibody 9431||20 µl||
|Phospho-c-Raf (Ser259) Antibody 9421||20 µl||
||H M R Mk X||74||Rabbit|
|c-Raf (D5X6R) Mouse mAb 12552||20 µl||
||H M R Mk B Pg||75||Mouse IgG1|
|Phospho-B-Raf (Ser445) Antibody 2696||20 µl||
||H M R Mk||86||Rabbit|
|B-Raf (55C6) Rabbit mAb 9433||20 µl||
||H M R Mk||86||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
|Anti-mouse IgG, HRP-linked Antibody 7076||100 µl||
The phospho-specific polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser299 of human A-Raf, Ser445 of human B-Raf and Ser259, 289, 296 and 301 of c-Raf. The total polyclonal antibody is produced by immunizing rabbits with a synthetic peptide corresponding to residues close to the linker domain of human A-Raf. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser338 of human c-Raf, a synthetic peptide corresponding to human B-Raf and a recombinant protein corresponding to residues in the middle of human c-Raf protein.
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