|H M R||Endogenous||80, 91||Rabbit IgG|
Western blot analysis of extracts from HeLa, NIH/3T3, and C2C12 cells using RanBP9 (D8M8D) Rabbit mAb.Learn more about how we get our images.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or RanBP9 siRNA (+), using RanBP9 (D8M8D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).Learn more about how we get our images.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised June 2016
Protocol Id: 263
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
RanBP9 (D8M8D) Rabbit mAb recognizes endogenous levels of total RanBP9 protein.
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His490 of human RanBP9 protein.
RanBP9 (RanBPM) is a Ran binding protein ubiquitously expressed and highly conserved in different organisms. It is a scaffolding protein that was identified as an interacting protein of MET inducing Ras- Erk activation (1). Several domains and motifs regulate the nuclear and cytoplasmic localization of RanBP9 (2). Research studies indicate that RanBP9 might contribute to the pathogenesis of Alzheimer’s Disease (AD). In addition to elevated expression levels in AD samples, this may involve interaction of RanBP9 with APP and BACE1 promoting endocytosis of APP and increased BACE1 mediated cleavage of APP to generate β-amyloid peptides (3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalSilence is a registered trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.
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|14638S||100 µl||$ 255.0|