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  3. PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit

PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit #9300

Inquiry Info. # 9300

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    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit provides reagents and protocols for the rapid analysis of Rb phosphorylation.

    Specificity / Sensitivity

    Phospho-Rb (Ser780, Ser795, Ser807/811) Antibodies detect endogenous levels of Rb only when phosphorylated at the target sites. The monoclonal Rb antibody does not recognize the Rb homologues p107 or p130, or other proteins.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser780, Ser795 or Ser807/811 of human Rb. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a fusion protein (Rb-C Fusion Protein #6022) containing residues 701-928 of human Rb .

    Background

    The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

    Alternate Names

    p105-Rb; pp110; retinoblastoma

    Database Links

    UniProt ID: P06400


    Entrez-Gene Id: 5925


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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