Render Target: STATIC
Render Timestamp: 2024-12-12T10:39:39.124Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:07.473
Product last modified at: 2024-12-03T11:15:38.695Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM4 (E1Y2G) Rabbit mAb #61689

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RBM4 recognizes endogenous levels of total RBM4 protein. This antibody does not cross-react with RBM4B protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro317 of human RBM4 protein.

    Background

    RBM4 functions as an RNA-binding protein and precursor mRNA splicing factor that antagonizes activity of PTBP1-mediated splicing (1-4). Upon phosphorylation at Ser309, RBM4 accumulates in stress granules and suppresses cap-dependent translation through eIF4A (3). Exon skipping of MEF2C by RBM4 has been shown to play a key role in the differentiation of brown adipocytes (5). The splicing activities of RBM4 seem to suppress tumorigenesis in the context of multiple cancer types (5-8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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