RBM5 (E8G8K) Rabbit mAb #86425
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
RBM5 (E8G8K) Rabbit mAb recognizes endogenous levels of total RBM5 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly325 of human RBM5 protein.
Background
RBM5 is an RNA binding protein that regulates alternative splicing through association with the spliceosome (1,2). First identified as a tumor suppressor gene, RBM5 interacts with U2AF65 in order to regulate many pro-apoptotic genes, such as Fas (3). RBM5 is notably deleted in many lung cancers, and also has been shown to be a key regulator in male germ cell pre-mRNA splicing (4-6).
- Deckert, J. et al. (2006) Mol Cell Biol 26, 5528-43.
- Fushimi, K. et al. (2008) Proc Natl Acad Sci U S A 105, 15708-13.
- Bonnal, S. et al. (2008) Mol Cell 32, 81-95.
- Shao, C. et al. (2012) World J Surg Oncol 10, 160.
- Li, P. et al. (2012) Cancer Epidemiol 36, 481-9.
- O'Bryan, M.K. et al. (2013) PLoS Genet 9, e1003628.
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