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  3. Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 )

Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 ) #5314

Inquiry Info. # 5314

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    Product Information

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF120. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

    Storage

    Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
    Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 20-26
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF120. All lots are greater than 98% pure.
    Endotoxin Less than 0.01 ng endotoxin/1μg mVEGF120.
    Activity The bioactivity of recombinant mVEGF120 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.
    Molecular Formula Recombinant mVEGF120 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,071. DTT-reduced protein migrates as a 20-26 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-37 kDa protein. The expected amino-terminal APTTE of recombinant hVEGF120 was verified by amino acid sequencing.

    Source / Purification

    Recombinant mouse VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428) was expressed in human 293 cells at Cell Signaling Technology.

    Background

    VEGF120 , a splice variant of the VEGF-A gene, is the murine counterpart to human VEGF121 (1,2). VEGF120 is produced by endothelial cells, macrophages, T cells, and others. VEGF120 is involved in angiogenesis, vascular permeability, vascular endothelial cell survival, growth, and migration (1). VEGF120 expression is induced by hypoxia, inflammatory cytokines, and through oncogene products in tumors (1,2). VEGF120 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF120 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/Akt, p38, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

    Alternate Names

    mVEGF; VEGF

    Database Links

    UniProt ID: Q00731


    Entrez-Gene Id: 22339


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