Render Target: STATIC
Render Timestamp: 2025-03-19T10:50:36.917Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:10:42.593
Product last modified at: 2025-02-10T19:30:09.368Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

RecQL5 (1A2) Mouse mAb #5847

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RecQL5 (1A2) Mouse mAb recognizes endogenous levels of total RecQL5 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human RecQL5 protein.

    Background

    The RecQ family is a group of DNA helicases that play an important role in global genomic stability (1). Mutations in three of the five known human RecQ proteins (BLM, WRN, and RECQL4) give rise to clinically distinct disorders that are characterized by features such as premature aging and predisposition to cancer (2,3). The clinical distinction of each disease associated with these mutations points to distinct roles that members of this helicase family play in DNA metabolism. The RecQL5 helicase has not yet been associated with any human disease, but RecQL5 -/- mice exhibit an increased incidence of cancer (4,5). It has recently been shown that RecQL5 protects genome stability through two parallel mechanims: helicase action and interaction with the initiation form of RNA Polymerase II (6). It has also been shown that RecQL5 -/- mouse embryonic stem cells display an elevated frequency of sister chromatic exchange (SCE), suggesting a role in suppression of homologous recombination and/or crossover events (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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