Render Target: STATIC
Render Timestamp: 2024-11-12T09:52:37.079Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:49.699
Product last modified at: 2024-09-30T08:00:55.065Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RGS14 (E4N2Q) Rabbit mAb #76703

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 65
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:3200 - 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #74575.

    Protocol

    Specificity / Sensitivity

    RGS14 (E4N2Q) Rabbit mAb recognizes endogenous levels of total RGS14 protein. Non-specific staining in fixed frozen mouse kidney may be observed by immunofluorescence.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse RGS14 protein.

    Background

    Regulator of G-protein signaling (RGS) proteins are a structurally diverse family of more than 30 member proteins enriched in brain that typically limit neurotransmitter receptor- and G protein-linked signaling in the CNS and elsewhere by serving as GTPase-activating proteins (GAPs) (1). RGS14 is a multifunctional signaling protein that regulates post-synaptic plasticity in neurons and acts as a scaffolding protein that integrates G protein, Ras/ERK, and calcium/calmodulin signaling pathways essential for spine plasticity and cell signaling (2). It is highly enriched within pyramidal cells of hippocampal area CA2, allowing RGS14 to naturally suppress long-term potentiation (LTP) and structural plasticity in that region (2-4). RGS14 has been shown to directly interact with two key Ca2+-activated proteins in CA2 neurons: calcium/calmodulin and CaMKII (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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