Render Target: STATIC
Render Timestamp: 2024-10-03T10:24:33.041Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RNase L (D4B4J) Rabbit mAb #27281

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Protocol

    Specificity / Sensitivity

    RNAse L (D4B4J) Rabbit mAb recognizes endogenous levels of total RNase L protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro717 of human RNase L protein.

    Background

    RNase L is an antiviral protein that is expressed in most mammalian cells (1). Latent RNase L in the cytoplasm is activated by the second messenger 2’,5’-linked oligoadenylate (2-5A), which is produced by oligoadenylate synthase (OAS) after it binds viral double-stranded RNA (dsRNA) (2, 3). RNase L forms a crossed homodimer that is stabilized by kinase homology and ankyrin domains, which position two kinase extension nuclease domains for RNA recognition (4). RNase L then degrades both viral and cellular RNA (5). In mouse models, RNase L has been shown to produce small self-RNAs that act to amplify innate antiviral immunity through IFN-β induction (6). Research has also shown that RNase L forms a complex with Filamin A that acts as a barrier to restrict virus entry, and that RNase L can induce autophagy in response to viral infection (7, 8). Finally, research suggests RNase L may contribute to type I diabetes onset through immune response regulation (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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