Render Target: STATIC
Render Timestamp: 2025-02-12T11:02:03.203Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2025-01-21 23:01:16.768
Product last modified at: 2025-02-05T09:00:24.489Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RTN3 (F5X9D) Rabbit mAb #59460

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RTN3 (F5X9D) Rabbit mAb recognizes endogenous levels of total RTN3 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RTN3 protein.

    Background

    Reticulon-3 (RTN3), a member of the reticulon protein family, plays a crucial role in maintaining the tubular shape and structural integrity of the endoplasmic reticulum (ER) network (1,2). The longest isoform of RTN3 (Rtn3L) carries six active LC3-interacting region domains, which are essential for LC3/GABARAP binding, fragmentation of ER tubules, and its function as an ER-phagy receptor (3,4). Rtn3L is also recruited to ER-endosome membrane contact sites by the endosomal protein Rab9a, where it promotes endosome maturation and cargo sorting (5). RTN3 is highly expressed in neuronal tissues and is a negative modulator of β-amyloid converting enzyme 1 (BACE1), the enzyme that cleaves amyloid precursor protein (APP) to release β-amyloid peptides (6). Therefore, altered expression of RTN3 may modulate amyloid plaque formation, a hallmark of Alzheimer's disease (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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