RUFY3 (E6H5Z) Rabbit mAb #74223

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	53, 54
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RUFY3 (E6H5Z) Rabbit mAb recognizes endogenous levels of total RUFY3 protein. This antibody detects multiple RUFY3 isoforms reported to be expressed by alternative splicing, including human RUFY3 isoform 1 and 2.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His58 of human RUFY3 protein.

Background

RUN and FYVE domain-containing protein 3 (RUFY3) is an actin-binding protein that is enriched in the brain. RUFY3 was identified as a protein that is upregulated during neuronal polarization, which is important for proper neuron functioning (1). RUFY3 likely functions in regulating neuronal polarization by interacting with the neuronal cytoskeleton via actin filament-binding proteins, such as Fascin (2). RUFY3 undergoes various forms of posttranslational modifications, including CASP3-dependent cleavage and phosphorylation, which may modulate selective synaptic and axonal degeneration during brain development and neurodegenerative disease (3).

Database Links

UniProt ID: Q7L099

Entrez-Gene Id: 22902

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For Research Use Only. Not For Use In Diagnostic Procedures.

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