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34425
S100A9 (D5O6O) Rabbit mAb (IHC Formulated)

S100A9 (D5O6O) Rabbit mAb (IHC Formulated) #34425

APPLICATIONS

REACTIVITY SENSITIVITY MW (kDa) Isotype
H Endogenous Rabbit IgG
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using S100A9 (D5O6O) Rabbit mAb (IHC Formulated).

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IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using S100A9 (D5O6O) Rabbit mAb (IHC Formulated) performed on the Leica® Bond™ Rx.

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IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using S100A9 (D5O6O) Rabbit mAb (IHC Formulated).

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IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using S100A9 (D5O6O) Rabbit mAb (IHC Formulated).

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IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using S100A9 (D5O6O) Rabbit mAb (IHC Formulated).

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IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using S100A9 (D5O6O) Rabbit mAb (IHC Formulated).

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Immunohistochemistry (Leica® Bond)

NOTE: Please see product datasheet or product webpage for appropriate antibody dilution.

  Step Reagents Time/Temperature

1.

Dewax

Bond Dewax Solution, 100% Alcohol, Bond Wash Solution

Pre-programmed Leica®Bond

2.

Antigen Retrieval

Bond Epitope Retrieval ER2 Solution

20 min., 100°C

3.

Peroxide Blocking

Peroxide Block*

5 min.

 

WASH

Bond Wash Solution

3x 0:00 min.

4.

Protein Block

CST #5425 NGS or #15019 Animal-Free Blocking Solution

20 min.

5.

Primary Antibody

Dilute in #8112 Antibody Diluent

30 min.

 

WASH

Bond Wash Solution

3x 2:00 min.

6.

Secondary Detection

Novolink Polymer*

10 min.

 

WASH

Bond Wash Solution/Deionized Water

Custom (see below)

7a.

Visualization

Mixed DAB Refine*

0:00 min.

7b.

Visualization

Mixed DAB Refine*

10 min.

 

WASH

Deionized Water

3x 0:00 min.

8.

Counterstain

Hematoxylin*

5 min.

 

WASH

Deionized Water

3x 0:00 min.

 

WASH

Bond Wash Solution

3x 0:00 min.

 

WASH

Deionized Water

3x 0:00 min.

9.

Dehydration (Offline):
Incubate sections in 95% ethanol two times for 10 sec. each.
Repeat in 100% ethanol, incubating sections two times for 10 sec each.
Repeat in xylene, incubating sections two times for 10 sec each.
Mount sections with coverslips and mounting medium (#14177).

  Custom wash: Bond Wash Solution 2:00
    Bond Wash Solution Dispenser Type: OPEN 0:00
    Bond Wash Solution 2:00
    Bond Wash Solution Dispenser Type: OPEN 0:00
    Bond Wash Solution 0:00
    Deionized Water 0:00

*Reagent included in Bond Polymer Refine Detection Kit
Catalog No: DS9800

LEICA® is a registered trademark of Leica®Microsystems IR GmbH.

Bond is a trademark of Leica®Biosystems Melbourne Pty. Ltd.

No affiliation or sponsorship between CST and Leica®Microsystems IR GmbH or Leica®Biosystems Melbourne Pty. Ltd. is implied.

posted February 2017

Protocol Id: 1444

Immunohistochemistry (Paraffin)

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. Xylene.
  2. Ethanol, anhydrous denatured, histological grade (100% and 95%).
  3. Deionized water (dH2O).
  4. Hematoxylin (optional).
  5. Wash Buffer:
    1. 1X Tris Buffered Saline with Tween® 20 (TBST): To prepare 1L 1X TBST add 100 ml 10X Tris Buffered Saline with Tween® 20 (#9997) to 900 ml dH20, mix.
  6. SignalStain® Antibody Diluent (#8112).
  7. 1X Citrate Unmasking Solution: To prepare 250 mL of 1X citrate unmasking solution, dilute 25 ml of SignalStain® Citrate Unmasking Solution (10X) (#14746) with 225 mL of dH2O.
  8. 3% Hydrogen Peroxide: To prepare 100 ml, add 10 ml 30% H2O2 to 90 ml dH2O.
  9. Blocking Solution: TBST/5% Normal Goat Serum or 1X Animal-Free Blocking Solution.
    1. TBST/5% Normal Goat Serum: to 5 ml 1X TBST, add 250 µl Normal Goat Serum (#5425).
    2. 1X Animal-Free Blocking Solution: to 4 mL of dH2O add 1 ml of Animal-Free Blocking Solution (5X) (#15019).
  10. Detection System: SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114).
  11. Substrate: SignalStain® DAB Substrate Kit (#8059).
  12. Hematoxylin: Hematoxylin (#14166).
  13. Mounting Medium: SignalStain® Mounting Medium (#14177).

B. Deparaffinization/Rehydration

NOTE: Do not allow slides to dry at any time during this procedure.

  1. Deparaffinize/hydrate sections:
    1. Incubate sections in three washes of xylene for 5 min each.
    2. Incubate sections in two washes of 100% ethanol for 10 min each.
    3. Incubate sections in two washes of 95% ethanol for 10 min each.
  2. Wash sections two times in dH2O for 5 min each.

C. Antigen Unmasking

For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.

D. Staining

  1. Wash sections in dH2O three times for 5 min each.
  2. Incubate sections in 3% hydrogen peroxide for 10 min.
  3. Wash sections in dH2O two times for 5 min each.
  4. Wash sections in wash buffer for 5 min.
  5. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature.
  6. Remove blocking solution and add 100–400 µl primary antibody diluted in SignalStain® Antibody Diluent (#8112) to each section. Incubate overnight at 4°C.
  7. Equilibrate SignalStain® Boost Detection Reagent (HRP, Rabbit #8114) to room temperature.
  8. Remove antibody solution and wash sections with wash buffer three times for 5 min each.
  9. Cover section with 1–3 drops SignalStain® Boost Detection Reagent (HRP, Rabbit #8114) as needed. Incubate in a humidified chamber for 30 min at room temperature.
  10. Wash sections three times with wash buffer for 5 min each.
  11. Add 1 drop (30 µl) SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent and mix well before use.
  12. Apply 100–400 µl SignalStain® DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.
  13. Immerse slides in dH2O.
  14. If desired, counterstain sections with hematoxylin (#14166).
  15. Wash sections in dH2O two times for 5 min each.
  16. Dehydrate sections:
    1. Incubate sections in 95% ethanol two times for 10 sec each.
    2. Repeat in 100% ethanol, incubating sections two times for 10 sec each.
    3. Repeat in xylene, incubating sections two times for 10 sec each.
  17. Mount sections with coverslips and mounting medium (#14177).

posted February 2010

revised March 2016

Protocol Id: 283

Application Dilutions
IHC-Leica® Bond™ 1:800
Immunohistochemistry (Paraffin) 1:400
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

S100A9 (D6O5O) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total S100A9 protein.

Species Reactivity:

Human

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human S100A9 protein.

S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1, 2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9).

  1. Odink, K. et al. Nature 330, 80-2.
  2. Edgeworth, J. et al. (1991) J Biol Chem 266, 7706-13.
  3. Rammes, A. et al. (1997) J Biol Chem 272, 9496-502.
  4. Steinbakk, M. et al. (1990) Lancet 336, 763-5.
  5. Ryckman, C. et al. (2003) J Immunol 170, 3233-42.
  6. Viemann, D. et al. (2005) Blood 105, 2955-62.
  7. Sinha, P. et al. (2008) J Immunol 181, 4666-75.
  8. Schonthaler, H.B. et al. (2013) Immunity 39, 1171-81.
  9. Lim, S.Y. et al. (2016) Oncogene 35, 5735-5745.
Entrez-Gene Id
6280
Swiss-Prot Acc.
P06702
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.

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