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8207
PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet #8207

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other Image 1 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb compared to a nonspecific negative control antibody (red).

other Image 2 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Confocal immunofluorescent analysis of mouse brain using S6 Ribosomal Protein (5G10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 3 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal protein (Ser235/Ser236) (D57.2.2E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 4 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (5G10) Rabbit mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 5 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of frozen U-87MG xenograft using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 6 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 7 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis using Phospho-S6 Ribosomal Protein (S235/236) (D57.2.2E) XP® Rabbit mAb on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right).

other Image 8 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 9 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).

other Image 10 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 11 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 12 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 13 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 14 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 15 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 16 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using S6 Ribosomal Protein (5G10) Rabbit mAb.

other Image 17 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded mouse spleen using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 18 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Immunohistochemical analysis of paraffin-embedded A549 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb.

other Image 19 - PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet

Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

To Purchase # 8207S
Product # Size Price
8207S
1 Kit $ 537

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb 4858 100 µl H M R Mk Mi Sc 32 Rabbit IgG
S6 Ribosomal Protein (5G10) Rabbit mAb 2217 100 µl H M R Mk 32 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.