Render Target: STATIC
Render Timestamp: 2024-10-04T09:38:43.604Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SARS-CoV-2 2'-O-ribose Methyltransferase (E6N2C) Rabbit mAb #19595

Filter:
  • WB

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Transfected Only
    MW (kDa) 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 2'-O-ribose Methyltransferase (E6N2C) Rabbit mAb recognizes transfected levels of total SARS-CoV-2 2'-O-ribose methyltransferase protein.

    Species Reactivity:

    Virus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of SARS-CoV-2 2'-O-ribose methyltransferase protein.

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2).

    SARS-CoV-2 2′-O-ribose methyltransferase, or 2′-O-MTase, is a non-structural protein (Nsp16) that is translated from ORF1b. 2′-O-ribose methyltransferase activity along with its co-factor Nsp10, is critically responsible for the formation of a cap on viral RNAs. The viral RNA cap resembles the cap of mRNAs in eukaryotic cells, and provides the viral RNA stability, effective translation, and protection from cellular innate immunity (3). The essential function of this non-structural protein in viral replication has made it an attractive target for potential antiviral strategies (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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