Render Target: STATIC
Render Timestamp: 2024-11-08T09:54:17.889Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-10-29 22:01:06.886
Product last modified at: 2024-11-04T09:00:11.588Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SART3 (F8W1S) Rabbit mAb #30270

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 135
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SART3 (F8W1S) Rabbit mAb recognizes endogenous levels of total SART3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala954 of human SART3 protein.

    Background

    Squamous cell carcinoma antigen recognized by T cells 3 (SART3), also known as p110 or Tip110, is an RNA binding protein that binds to the U4/U6 small nuclear ribonucleoprotein complex during the recycling phase of the spliceosome cycle (1,2). SART3 mediates recruitment of USP4 to deubiquitinate PRPF3, leading to the disassembly of U4 and U6 components (3). Additionally, SART3 can target USP15 to regulate histone H2B deubiquitination (4). SART3 has also been shown to play a role in hematopoiesis regulation and pluripotency, where it controls mRNA splicing of OCT4 (5-7). During HIV-1 infection, SART3 binds to and activates the viral Tat protein, promoting viral replication (8,9). SART3 is highly expressed in many tumor tissues, and various peptides have been used to generate cytotoxic T lymphocytes for targeted immunotherapy (10-13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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