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SASH3/SLY (F5S3X) Rabbit mAb #77860

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  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SASH3/SLY (F5S3X) Rabbit mAb recognizes endogenous levels of total SASH3/SLY protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro53 of human SASH3/SLY protein.

    Background

    Encoded by an X-linked gene, sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY), is a putative lymphoid-specific signaling adaptor (1). One of the first clues that SASH3 plays a role in regulating the biology of lymphoid cells arose from research studies demonstrating that SASH3 is modified by phosphorylation subsequent to T cell receptor (TCR) engagement (2). Furthermore, the presence of SAM and SH3 domains strongly suggests that SASH3/SLY participates in protein-protein interactions to regulate immune cell effector functions downstream of antigen receptor engagement. Indeed, naturally occurring loss-of-function mutations have been identified in human SASH3/SLY that blunt activation of multiple key signaling nodes following TCR engagement, leading to an impairment of T cell proliferation and survival (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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