Interested in promotions? | Click here >>
89194
SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free) #89194

Reviews ()
Citations (0)
Filter:
  1. IHC
Immunohistochemistry Image 1: SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific). Data were generated using the standard formulation of this product.
To Purchase # 89194
Cat. # Size Qty. Price
89194SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #97958. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #97958.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

SDF1/CXCL12 (D8G6H) Rabbit mAb (IHC-Specific) (BSA and Azide Free) recognizes endogenous levels of total SDF1/CXCL12 protein by immunohistochemistry on paraffin-embedded tissue sections. This antibody recognizes both SDF1/CXCL12α and SDF1/CXCL12β.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SDF1/CXCL12 protein.

Background

The stromal cell derived factor 1 (SDF1/CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1/CXCL12 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1/CXCL12 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1/CXCL12 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1/CXCL12 isoforms (SDF1/CXCL12α and SDF1/CXCL12β) that have different properties and biological activities (7). Additional isoforms of SDF1/CXCL12 have been reported (8,9).
  1. Maekawa, T. and Ishii, T. (2000) Intern Med 39, 90-100.
  2. Ray, N. and Doms, R.W. (2006) Curr Top Microbiol Immunol 303, 97-120.
  3. Sugiyama, T. et al. (2006) Immunity 25, 977-88.
  4. Ara, T. et al. (2003) Immunity 19, 257-67.
  5. Kucia, M. et al. (2004) J Mol Histol 35, 233-45.
  6. Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-24.
  7. De La Luz Sierra, M. et al. (2004) Blood 103, 2452-9.
  8. Gleichmann, M. et al. (2000) Eur J Neurosci 12, 1857-66.
  9. Yu, L. et al. (2006) Gene 374, 174-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.