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Sec31A Antibody #8506

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Inquiry Info. # 8506

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    Product Specifications

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 95-140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Sec31A Antibody recognizes endogenous levels of multiple isoforms of total Sec31A protein, ranging in sizes of 95-140 kDa.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro810 of human Sec31A protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).
    Multiple isoforms of Sec31A protein have been reported (10).

    Alternate Names

    ABP125; ABP130; DKFZp686N07171; HSPC275; HSPC334; KIAA0905; MGC90305; NEDSOSB; Protein transport protein Sec31A; protein-transport protein SEC31; SC31A; SEC31 homolog A (S. cerevisiae); SEC31 homolog A, COPII coat complex component; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; SEC31A; SEC31L1; Web1-like protein; yeast Sec31p homolog

    For Research Use Only. Not for Use in Diagnostic Procedures.
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