Semaphorin-4A (E5N3K) Rabbit mAb (BSA and Azide Free) #35176

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	90
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #57331. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #57331

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Semaphorin-4A (E5N3K) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Semaphorin-4A protein. An unknown background band is detected at 55 kDa in some cell lines.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu756 of human Semaphorin-4A protein.

Background

Semaphorin-4A, or Sema4A, is a membrane-type class IV semaphorin family member glycoprotein and is expressed in dendritic cells, B cells, T cells, and endothelial cells (1). The protein structure consists of an N-terminus signal peptide, a sema domain, a C2 type Ig domain, a transmembrane region, and a cytoplasmic tail (2). Semaphorin-4A exerts diverse biological effects through several different receptor-mediated pathways and plays important roles in immunity, angiogenesis, neuronal migration, and tumor progression. Semaphorin-4A enhances T cell activation by interacting with TIM-2, and T cell-derived Semaphorin-4A plays a role in helper T cell (Th) differentiation (3,4). In regulatory T cells (Tregs), Semaphorin-4A plays an important role in enhancing stability through interactions with neuropilin-1 (Nrp1) (5). Semaphorin-4A interacts with PlexinD1, which is expressed in endothelial cells and negatively regulates angiogenesis (6). In presynaptic neurons, Semaphorin-4A promotes the development of inhibitory or excitatory synapses through interactions with PlexinB1 or PlexinB2, respectively (7). Semaphorin-4A has been identified as a therapeutic target to promote antitumor responses, and its expression in the tumor microenvironment (TME) has been suggested as a biomarker to predict response to immunotherapy (8).

Database Links

UniProt ID: Q9H3S1

Entrez-Gene Id: 64218

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

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