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  1. Products /
  2. Primary Antibodies /
  3. Semaphorin-7A (F2L1S) Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Semaphorin-7A (F2L1S) Rabbit mAb #45588

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  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Semaphorin-7A (F2L1S) Rabbit mAb recognizes endogenous levels of total semaphorin-7A protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro400 of human Semaphorin-7A protein.

    Background

    Semaphorin-7A is a GPI-anchored membrane protein that plays important roles in neuronal development and immune responses via integrin-mediated signaling. It promotes axon growth by activating the mitogen-activated protein kinase (MAPK) pathway through β1 integrin receptors. When expressed on activated T cells, it promotes the production of proinflammatory cytokines by macrophages through the α1β1 integrin receptor (1). Semaphorin-7A may also play a role in resolving inflammation through its regulation of macrophage chemotaxis and chemokinesis, and by promoting macrophage differentiation and polarization toward an anti-inflammatory phenotype (2). Neuroinflammation is a detrimental component of neurodegenerative diseases, such as Parkinson’s disease (PD) and multiple sclerosis (MS). The role that semaphorin-7A plays in mediating inflammatory responses in microglia in the brain suggests that it may be a therapeutic target for these diseases. In a cell model of neuroinflammation, semaphorin-7A was shown to promote cell viability and survival through its mediation of MAPK and peroxisome proliferator-activated receptor γ (PPARγ) pathways (3).

    Database Links

    UniProt ID: O75326


    Entrez-Gene Id: 8482


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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