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38461
Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #38461

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Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 1

Flow cytometric analysis of THP-1 cells, untreated (blue) or LPS-treated (100 ng/ml, 3 hr; green), using IL-1β (D3U3E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 2

Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using RANTES (R40) Antibody.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 3

Western blot analysis of recombinant human CXCL10 protein using CXCL10 (D5L5L) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 4

Immunoprecipitation of IL-6 from NCI-H460 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D3K2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D3K2N) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 5

Western blot analysis of extracts from the media of THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; overnight), using TNF-α (D5G9) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines using MMP-3 (D7F5B) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 7

Immunoprecipitation of MCP-1 protein from conditioned medium of A549 cells treated with TNF-α (20 ng/ml, 6 hr). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MCP-1 Antibody (Carboxy-terminal Antigen). Western blot analysis was performed using MCP-1 Antibody (Carboxy-terminal Antigen).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 8

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 9

Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 10

Western blot analysis of HeLa cells, mock transfected or transfected with human RANTES construct, using RANTES (R40) Antibody.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 11

Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using CXCL10 (D5L5L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 12

Western blot analysis of extracts from COS-7 and HUVEC cells using PAI-1 (D9C4) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 13

Western blot analysis of 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 using IL-6 (D3K2N) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 14

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; various time points), using TNF-α (D5G9) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 15

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human MCP-1 protein (hMCP-1; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-actin (D6A8) Rabbit mab #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 16

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 17

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (100 ng/ml, 6 hr; +) and treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +), using IL-6 (D3K2N) Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 18

Western blot analysis of recombinant human TNF-α #8902 using TNF-α (D5G9) Rabbit mAb (left) or TNF-α Antibody #3707 (right).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 19

Western blot analysis of extracts from A549 cells, untreated (-) or treated with TNF-α (20 ng/ml, 6 hr; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 20

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

To Purchase # 38461T
Product # Size Price
38461T
1 Kit  (8 x 20 µl) $ 554

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
IL-1β (D3U3E) Rabbit mAb 12703 20 µl
  • WB
  • IF
  • F
H 17, 31 Rabbit IgG
RANTES (R40) Antibody 2987 20 µl
  • WB
H 10 Rabbit 
CXCL10 (D5L5L) Rabbit mAb 14969 20 µl
  • WB
H 10 Rabbit IgG
PAI-1 (D9C4) Rabbit mAb 11907 20 µl
  • WB
H Mk B 48 Rabbit IgG
IL-6 (D3K2N) Rabbit mAb 12153 20 µl
  • WB
  • IP
H 21-28 Rabbit IgG
TNF-α (D5G9) Rabbit mAb 6945 20 µl
  • WB
  • IP
H 18, 25 Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351 20 µl
  • WB
H R 60 Rabbit IgG
MCP-1 Antibody (Carboxy-terminal Antigen) 39091 20 µl
  • WB
  • IP
H 13-15 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein.

CXCL10 (D5L5L) Rabbit mAb also cross-reacts with a 100kDa protein of unknown origin in some cell lines.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Arg294 of human PAI-1 protein, residues surrounding Ser417 of human MMP3 protein, recombinant human IL-1β protein, recombinant human CXCL10 protein, recombinant human IL-6 protein, and recombinant human TNF-α protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES and near the carboxy terminus of human MCP-1 protein.

Background

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.

  1. Tchkonia, T. et al. (2013) J Clin Invest 123, 966-72.
  2. Sun, Y. et al. (2018) Trends Mol Med 24, 871-885.
  3. Rao, S.G. and Jackson, J.G. (2016) Trends Cancer 2, 676-687.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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