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38461
Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit
Primary Antibodies

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #38461

Western Blotting Image 1

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using RANTES (R40) Antibody.

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Western Blotting Image 3

Western blot analysis of recombinant human CXCL10 protein using CXCL10 (D5L5L) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from COS-7 and HUVEC cells using PAI-1 (D9C4) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 using IL-6 (D3K2N) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from the media of THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; overnight), using TNF-α (D5G9) Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using MMP3 (D7F5B) Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human MCP-1 protein (hMCP-1; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-actin (D6A8) Rabbit mab #8457 (lower).

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 10

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

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Western Blotting Image 11

Western blot analysis of HeLa cells, mock transfected or transfected with human RANTES construct, using RANTES (R40) Antibody.

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Western Blotting Image 12

Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using CXCL10 (D5L5L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 13

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (100 ng/ml, 6 hr; +) and treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +), using IL-6 (D3K2N) Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 14

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; various time points), using TNF-α (D5G9) Rabbit mAb.

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Western Blotting Image 15

Western blot analysis of extracts from A549 cells, untreated (-) or treated with TNF-α (20 ng/ml, 6 hr; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Flow Cytometry Image 16

Flow cytometric analysis of THP-1 cells, untreated (blue) or LPS-treated (100 ng/ml, 3 hr; green), using IL-1β (D3U3E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

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IP Image 17

Immunoprecipitation of IL-6 from NCI-H460 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D3K2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D3K2N) Rabbit mAb.

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Western Blotting Image 18

Western blot analysis of recombinant human TNF-α #8902 using TNF-α (D5G9) Rabbit mAb (left) or TNF-α Antibody #3707 (right).

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IP Image 19

Immunoprecipitation of MCP-1 protein from conditioned medium of A549 cells treated with TNF-α (20 ng/ml, 6 hr). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MCP-1 Antibody (Carboxy-terminal Antigen). Western blot analysis was performed using MCP-1 Antibody (Carboxy-terminal Antigen).

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IF-IC Image 20

Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
IL-1β (D3U3E) Rabbit mAb 12703 20 µl
  • WB
  • IF
  • F
H 17, 31 Rabbit IgG
RANTES (R40) Antibody 2987 20 µl
  • WB
H 10 Rabbit 
CXCL10 (D5L5L) Rabbit mAb 14969 20 µl
  • WB
H 10 Rabbit IgG
PAI-1 (D9C4) Rabbit mAb 11907 20 µl
  • WB
H Mk B 48 Rabbit IgG
IL-6 (D3K2N) Rabbit mAb 12153 20 µl
  • WB
  • IP
H 21-28 Rabbit IgG
TNF-α (D5G9) Rabbit mAb 6945 20 µl
  • WB
  • IP
H 18, 25 Rabbit IgG
MMP3 (D7F5B) Rabbit mAb 14351 20 µl
  • WB
H R 60 Rabbit IgG
MCP-1 Antibody (Carboxy-terminal Antigen) 39091 20 µl
  • WB
  • IP
H 13-15 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein.

CXCL10 (D5L5L) Rabbit mAb also cross-reacts with a 100kDa protein of unknown origin in some cell lines.

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Arg294 of human PAI-1 protein, residues surrounding Ser417 of human MMP3 protein, recombinant human IL-1β protein, recombinant human CXCL10 protein, recombinant human IL-6 protein, and recombinant human TNF-α protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES and near the carboxy terminus of human MCP-1 protein.

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.

  1. Tchkonia, T. et al. (2013) J Clin Invest 123, 966-72.
  2. Sun, Y. et al. (2018) Trends Mol Med 24, 871-885.
  3. Rao, S.G. and Jackson, J.G. (2016) Trends Cancer 2, 676-687.
Entrez-Gene Id
6352 , 3627 , 3553 , 3569 , 6347 , 4314 , 5054 , 7124
Swiss-Prot Acc.
P13501 , P02778 , P01584 , P05231 , P13500 , P08254 , P05121 , P01375
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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