Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #38461
Product Information
Kit Usage Information
Protocols
- 2987: Western Blotting
- 6945: Western Blotting, Immunoprecipitation (Agarose)
- 7074: Western Blotting
- 11907: Western Blotting
- 12153: Western Blotting, Immunoprecipitation (Agarose)
- 12703: Western Blotting, Immunofluorescence, Flow
- 14351: Western Blotting
- 14969: Western Blotting
- 39091: Western Blotting, Immunoprecipitation (Magnetic)
Product Description
Specificity / Sensitivity
Each antibody in the Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein.
CXCL10 (D5L5L) Rabbit mAb also cross-reacts with a 100kDa protein of unknown origin in some cell lines.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Arg294 of human PAI-1 protein, residues surrounding Ser417 of human MMP3 protein, recombinant human IL-1β protein, recombinant human CXCL10 protein, recombinant human IL-6 protein, and recombinant human TNF-α protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES and near the carboxy terminus of human MCP-1 protein.
Background
Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).
Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.
Limited Uses
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