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  1. Products /
  2. Primary Antibodies /
  3. Separase (F4R3M) Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Separase (F4R3M) Rabbit mAb #50574

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  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 233, 160
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Separase (F4R3M) Rabbit mAb recognizes endogenous levels of total separase protein. This antibody is expected to detect full-length isoform 1 and the N-terminal cleavage fragment. This antibody is not expected to detect the C-terminal cleavage fragment. Based on the peptide antigen sequence, this antibody is expected to recognize isoform 2. This antibody detects an approximately 40 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1320 of human separase protein.

    Background

    Caspase-like protein ESPL1 (separase), also known as separin, is a protease critical for chromosome segregation. It plays a role in spindle stabilization and elongation, apoptosis, and DNA repair. Separase cleaves the SCC1/RAD21 subunit of the cohesin complex at the start of anaphase, allowing sister chromatid segregation, an irreversible step in cell division (1). Separase is negatively regulated by securin, which inhibits its proteolytic activity, and by CDK1/cyclin B-dependent phosphorylation. Both securin and cyclin B are degraded via the anaphase-promoting complex/cyclosome (APC/C) (2,3). Dysregulation of separase activity is linked to cancer and genome instability, making it a target for cancer drug discovery (4,5). Separase is overexpressed in human cancer, and overexpression is thought to induce aneuploidy and tumorigenesis (1,6).

    Database Links

    UniProt ID: Q14674


    Entrez-Gene Id: 9700


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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