Render Target: STATIC
Render Timestamp: 2024-12-02T10:45:23.018Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:55:54.308
Product last modified at: 2024-09-30T08:01:12.509Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SH3BP4 (D3D9I) Rabbit mAb #55935

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SH3BP4 (D3D9I) Rabbit mAb recognizes endogenous levels of total SH3BP4 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human SH3BP4 protein.

    Background

    The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by enviromental factors such as energy levels, growth factors, and amino acids (3, 4). The GTPases RagA, RagB, RagC, and RagD mediate amino acid signaling to activate mTORC1 (1, 2). SH3BP4 (SH3 domain-binding protein 4) binds to the inactive Rag GTPase complex during amino acid starvation and prevents the association of Rag GTPase complex with mTORC1 resulting in the suppression of mTORC1 activation and cell growth (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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