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9793
SHP-2 Antibody Sampler Kit
Primary Antibodies

SHP-2 Antibody Sampler Kit #9793

Western Blotting Image 1

Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody (upper) or control SHP-2 Antibody #3752 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using SHP-2 (D50F2) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of total cell lysates from NIH/3T3 cells, serum-starved overnight and treated with PDGF (#9909) for 5 minutes, using Phospho-SHP-2 (Tyr580) Antibody (upper) or control SHP-2 Antibody #3752 (lower).

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Western Blotting Image 4

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-SHP-2 (Tyr542) Antibody 3751 20 µl
  • WB
  • IP
H M R 72 Rabbit 
SHP-2 (D50F2) Rabbit mAb 3397 20 µl
  • WB
  • IP
H M R Mk 72 Rabbit IgG
Phospho-SHP-2 (Tyr580) Antibody 3703 20 µl
  • WB
  • IP
H M R 72 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The SHP-2 Antibody Sampler Kit provides an economical means to evaluate levels of SHP-2 protein phosphorylated at the specified sites, as well as total SHP-2 levels. The kit contains enough primary and secondary antibody to perform two western blot experiments per antibody.

SHP-2 (D50F2) Rabbit mAb detects endogenous levels of total SHP-2 protein. Phospho- (Tyr542) SHP-2 Antibody detects endogenous SHP-2 only when phosphorylated at the specified at Tyr542. Phospho- SHP-2 (Tyr580) Antibody detects endogenous level of SHP2 only when phosphorylated at Tyr580. Some cross-reactivity with phosphorylated RTKs may occur. See individual product webpages for more information.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human SHP-2. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr542 and Tyr580 of human SHP-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).

  1. Qu, C.K. (2000) Cell Res 10, 279-88.
  2. Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25.
  3. Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72.
  4. Bennett, A.M. et al. (1994) Proc Natl Acad Sci U S A 91, 7335-9.
  5. Lu, W. et al. (2001) Mol Cell 8, 759-69.
  6. Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90.
Entrez-Gene Id
5781
Swiss-Prot Acc.
Q06124
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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