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Sin1 (D7G1A) Rabbit Monoclonal Antibody

Sin1 (D7G1A) Rabbit Monoclonal Antibody #12860

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Product Specifications

REACTIVITY	H R Mk
SENSITIVITY	Endogenous
MW (kDa)	74, 78
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Sin1 (D7G1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sin1 protein, including isoforms Sin1.1 and Sin1.2.

Species Reactivity:

Human, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Sin1 protein.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, rictor siRNA knockdown inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5).

Alternate Names

JC310; MAPK associated protein 1; MAPKAP1; MEKK2-interacting protein 1; MGC2745; MIP1; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; SIN1; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1

Database Links

UniProt ID: Q9BPZ7

Entrez-Gene Id: 79109

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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