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Slug (L40C6) Mouse mAb #9589

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Inquiry Info. # 9589

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Slug (L40C6) Mouse mAb detects endogenous levels of total Slug protein. This antibody does not recognize transfected human or mouse Snail proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein.

    Background

    Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).

    Alternate Names

    MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; SLUGH; SLUGH1; SNAI2; snail 2; snail family transcriptional repressor 2; snail family zinc finger 2; snail homolog 2; snail homolog 2 (Drosophila); Snail2; WS2D; Zinc finger protein SNAI2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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