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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb 3108 20 µl
Western Blotting
H M R Mi 60 Rabbit IgG
Smad2 (D43B4) XP® Rabbit mAb 5339 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 60 Rabbit IgG
Smad2/3 (D7G7) XP® Rabbit mAb 8685 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 52, 60 Rabbit IgG
Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb 9520 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R 52 Rabbit IgG
Smad3 (C67H9) Rabbit mAb 9523 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 52 Rabbit IgG
Smad4 (D3M6U) Rabbit mAb 38454 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 70 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Smad2/3 Antibody Sampler Kit provides an economical means of detecting target proteins of the TGF-β signaling pathway. The kit includes enough antibody to perform two western blots with each primary antibody.


Specificity / Sensitivity

Each antibody in the Smad2/3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total Smad2, Smad3, and Smad4 antibodies detect their respective targets at endogenous levels.


Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human Smad2 or Ser423/425 of human Smad3 protein. Total Smad2, Smad3, and Smad4 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino termini of mouse Smad2 and Smad3, surrounding His198 of human Smad2/3, or surrounding Asp165 of human SMAD4 protein.

Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smad proteins by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smad proteins regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-4).


1.  Horbelt, D. et al. (2012) Int J Biochem Cell Biol 44, 469-74.

2.  Ikushima, H. and Miyazono, K. (2010) Nat Rev Cancer 10, 415-24.

3.  Kitisin, K. et al. (2007) Sci STKE 2007, cm1.

4.  Schmierer, B. and Hill, C.S. (2007) Nat Rev Mol Cell Biol 8, 970-82.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

12747
Smad2/3 Antibody Sampler Kit