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52033
Small Cell Lung Cancer Biomarker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Small Cell Lung Cancer Biomarker Antibody Sampler Kit #52033

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Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 1
Simple Western™ analysis of lysates (1 mg/mL) from MCF-7 cells using YAP (D8H1X) XP® Rabbit mAb #14074. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 2
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H526 cells and either POU2F3 (E5N2D) XP® Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human TRPM5 intron 4 primers, human ASCL2 upstream primers, human GFI-B upstream primers, and human IGFBP2 Intron 1 primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 3
Confocal immunofluorescent analysis of WERI-Rb-1 (left, positive) and KARPAS 299 (right, negative) using NeuroD1 (D90G12) Rabbit mAb (green) and DAPI #4083 (blue).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines using ASCL1 (E5S4Q) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of ASCL1 protein in U-2 OS and SK-MEL-28 cells is consistent with the predicted expression pattern.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 5
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.  
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from TTF-1 positive H441 and TT cells, and TTF-1 negative A549 and HeLa cells, using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 7
Western blot analysis of extracts from control HeLa cells (Lane 1) or YAP knockout HeLa cells (Lane 2) using YAP (D8H1X) XP® Rabbit mAb (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the YAP knockout HeLa cells confirms the specificity of the antibody for YAP.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 8
Flow cytometric analysis of RL cells (blue, negative) and A-204 cells (green, positive) using YAP (D8H1X) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 9
Western blot analysis of extracts from mouse brain, rat brain, human brain, and mouse liver using Enolase-2 (E2H9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb performed on the Leica® BOND™ Rx.  
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 11
Western blot analysis of extracts from NCI-H526, MDA-MB-453, and HCT 116 cells using POU2F3 (E5N2D) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Expression levels of POU2F3 among cell lines are consistent with expectations based on publicly available bioinformatic databases, confirming specificity of the antibody for POU2F3.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 12
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from TT and NCI-H524 cells using DLL3 (E3J5R) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 14
Western blot analysis of extracts from TT, SH-SY5Y, and IMR-32 cells using CHGA (E8X7R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, IMR-32 cells have lower expression of CHGA protein.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 15
Western blot analysis of extracts from various cell lines and tissues using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 17
Confocal immunofluorescent analysis of the hypothalamic region of adult mouse brain using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 18
Western blot analysis of extracts from various cell lines using YAP (D8H1X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, RL cells are negative for YAP protein expression.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using Enolase-2 (E2H9X) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 20
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with plasmid encoding Myc/DDK-tagged POU2F3 protein (lane 2), using POU2F3 (E5N2D) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb performed on the Leica® BOND™ Rx.  
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 22
Immunoprecipitation of DLL3 protein from NCI-H524 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DLL3 (E3J5R) Rabbit mAb. Western blot analysis was performed using

DLL3 (E3J5R) Rabbit mAb.

Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 23
Confocal immunofluorescent analysis of SH-SY5Y cells (left, positive) and HuH-6 cells (right, negative) using CHGA (E8X7R) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 24
Immunoprecipitation of NCAM1 protein from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NCAM1 (CD56) (E7X9M) XP® Rabbit mAb. Western blot analysis was performed using NCAM1 (CD56) (123C3) Mouse mAb #3576. Anti-mouse IgG, HRP-linked Antibody #7076 was used as the secondary antibody.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 26
Confocal immunofluorescent analysis of H441 (left) and A549 (right) cells using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 27
Immunoprecipitation of YAP protein from A-204 cell extracts using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or YAP (D8H1X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using YAP (D8H1X) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 30
Western blot analysis of extracts from various human cell lines using NeuroD1 (D90G12) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using DLL3 (E3J5R) Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma with staining of peripheral nerve using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the gallbladder using ASCL1 (E5S4Q) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s lymphoma using YAP (D8H1X) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 35
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded human large cell neuroendocrine lung carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded TT cell pellet (left, high-expressing), NCI-H524 cell pellet (middle, low-expressing) or SK-BR-3 cell pellet (right, negative) using DLL3 (E3J5R) Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 39
Immunohistochemical analysis of paraffin-embedded human metastatic neuroendocrine carcinoma of the lung (two regions of interest) using ASCL1 (E5S4Q) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 40
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb performed on the Leica® BOND™ Rx.  
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 41
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 42
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma (left, positive) or non-small cell lung carcinoma (right, negative) using DLL3 (E3J5R) Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 44
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded mouse brain using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 48
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 49
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 50
Immunohistochemical analysis of paraffin-embedded NCI-H146 cell pellet (left, positive) or SK-MEL-28 cell pellet (right, negative) using ASCL1 (E5S4Q) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 51
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 52
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 53
Immunohistochemical analysis of paraffin-embedded normal human skin using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 54
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 55
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, wild-type (left, positive) or YAP/TAZ knockout (right, negative), using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 56
Immunohistochemical analysis of paraffin-embedded TT cell pellet (left, positive) or Huh7 cell pellet (right, negative) using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 57
Immunohistochemical analysis of paraffin-embedded normal human appendix using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 58
Immunohistochemical analysis of paraffin-embedded normal rhesus monkey spleen using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 59
Immunohistochemical analysis of paraffin-embedded BEN cell pellet, untransfected (left, YAP/TAZ low), YAP-transfected (middle) or TAZ-transfected (right), using YAP (D8H1X) XP® Rabbit mAb (top) or TAZ (E9J5A) XP® Rabbit mAb #72804 (bottom).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 60
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Enolase-2 (E2H9X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 61
Immunohistochemical analysis of paraffin-embedded normal human parathyroid gland using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 62
Immunohistochemical analysis of paraffin-embedded mouse ovary using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 63
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma (left), colon carcinoma case 1 (middle) or colon carcinoma case 2 (right), using YAP (D8H1X) XP® Rabbit mAb (top) or TAZ (E9J5A) XP® Rabbit mAb #72804 (bottom).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 64
Immunohistochemical analysis of paraffin-embedded normal human kidney using POU2F3 (E5N2D) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 65
Immunohistochemical analysis of paraffin-embedded mouse prostate using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 66
Immunohistochemical analysis of paraffin-embedded mouse small intesine using YAP (D8H1X) XP® Rabbit mAb (left) or YAP Antibody (right). These two antibodies detect unique, non-overlapping epitopes on YAP protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 67
Immunohistochemical analysis of paraffin-embedded NCI-H526 cell pellet (left, positive) or HCT 116 cell pellet (right, negative) using POU2F3 (E5N2D) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 68
Immunohistochemical analysis of paraffin-embedded mouse colon using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 69
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 70
Immunohistochemical analysis of paraffin-embedded endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) or NCAM (CD56) (123C3) Mouse mAb #3576 (right). These two antibodies detect independent, unique epitopes on human NCAM1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 71
Immunohistochemical analysis of paraffin-embedded PANC-1 (left) and RL (right) cell pellets using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 72
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 73
Immunohistochemical analysis of paraffin-embedded human benign prostatic hyperplasia using YAP (D8H1X) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 74
Immunohistochemical analysis of paraffin-embedded SH-SY5Y cell pellet (left, positive) or MCF7 cell pellet (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 75
Confocal immunofluorescent analysis of mouse spinal cord (left), or rat brainstem (right) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Tissue was mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 76
Confocal immunofluorescent analysis of SH-SY5Y cells (left, positive) or HeLa cells (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 77
Confocal immunofluorescent analysis of Neuro-2a cells (left, high-expressing) or C2C12 cells (right, low-expressing) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 78
Flow cytometric analysis of HeLa cells (blue) and SH-SY5Y cells (green) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 79
Confocal immunofluorescent analysis of low confluence MCF 10A cells (left), high confluence MCF 10A (center), and YAP negative RL cells (right) using YAP (D8H1X) XP® Rabbit mAb (green). Blue pseudocolor in lower images = DRAQ5® #4084 (fluorescent DNA dye). Increased nuclear localization of YAP protein is seen in low confluence (proliferating) cells.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 80
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H2052 cells and YAP (D8H1X) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across CTGF, a known target gene of YAP (see additional figure containing ChIP-qPCR data).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 81
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H2052 cells and YAP (D8H1X) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 6 (upper), including CTGF (lower), a known target gene of YAP (see additional figure containing ChIP-qPCR data).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 82
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-2052 cells and either YAP (D8H1X) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 intron 2 primers, and SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 83
CUT&RUN was performed with NCI-H2052 cells and YAP (D8H1X) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across TSPAN2, a known target gene of YAP (see additional figure containing CUT&RUN-qPCR data).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 84
CUT&RUN was performed with NCI-H2052 cells and YAP (D8H1X) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across across chromosome 1 (upper), including TSPAN2 (lower), a known target gene of YAP (see additional figure containing CUT&RUN-qPCR data).
Small Cell Lung Cancer Biomarker Antibody Sampler Kit: Image 85
CUT&RUN was performed with NCI-H2052 cells and either YAP (D8H1X) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human C1D downstream primers, human TSPAN2 upstream primers, and human ITM2A upstream primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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To Purchase # 52033
Cat. # Size Qty. Price
52033T		 1 Kit  (9 x 20 microliters)
$ 708

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
ASCL1 (E5S4Q) XP® Rabbit mAb 10585 20 µl
  • WB
  • IHC
 H 30 Rabbit IgG
NeuroD1 (D90G12) Rabbit mAb 7019 20 µl
  • WB
  • IP
  • IF
 H 49 Rabbit IgG
YAP (D8H1X) XP® Rabbit mAb 14074 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
  • C&R
 H M R Hm Mk 65-78 Rabbit IgG
POU2F3 (E5N2D) XP® Rabbit mAb 36135 20 µl
  • WB
  • IHC
  • ChIP
 H 45-60 Rabbit IgG
Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb 12373 20 µl
  • WB
  • IF
 H M R 39, 42 Rabbit 
DLL3 (E3J5R) Rabbit mAb 71804 20 µl
  • WB
  • IP
  • IHC
 H 65 Rabbit IgG
NCAM1 (CD56) (E7X9M) XP® Rabbit mAb 99746 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
 H M R Mk 120 to 220 Rabbit IgG
Enolase-2 (E2H9X) XP® Rabbit mAb 24330 20 µl
  • WB
  • IHC
 H M R 47 Rabbit IgG
CHGA (E8X7R) Rabbit mAb 85798 20 µl
  • WB
  • IF
 H 80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Small Cell Lung Cancer Biomarker Antibody Sampler Kit provides a means of detecting common biomarkers studied in small cell lung cancer (SCLC). The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Small Cell Lung Cancer Biomarker Antibody Sampler Kit detects endogenous levels of its target protein. Enolase-2 (E2H9X) XP® Rabbit mAb does not cross-react with human Enolase-1 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro83 of human ASCL1, Leu160 of human POU2F3, Gly315 of human NeuroD1, Pro566 of human DLL3, Pro799 of human NCAM1/CD56, residues near the amino terminus of human TTF-1 protein, residues near the carboxy terminus of human Enolase-2 and CHGA protein, and a recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence is 100% conserved among all known isoforms of human YAP protein.

Background

Lung cancer is the leading cause of cancer-related mortality worldwide (1). It is generally divided into two broad histological classifications: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). SCLC is particularly aggressive and has been further subdivided by biological heterogeneity. Subtypes of SCLC have recently been described based on expression distinct transcriptional regulators (2,3). These subtypes were labeled as SCLC-A expressing achaete-scute homolog 1 (ASCL1), SCLC-N expressing neurogenic differentiation factor 1 (NeuroD1), SCLC-Y expressing yes-associated protein 1 (YAP), and SCLC-P expressing POU class 2 homeobox 3 (POU2F3). ASCL1 and NeuroD1 drive a neuroendocrine phenotype through regulation of distinct genes. DLL3, an inhibitor of NOTCH signaling, is upregulated by ASCL1 (4). NCAM1 (neural cell adhesion molecule, CD56) is an adhesion glycoprotein that mediates neuronal attachment, neurite extension, and is a marker for the neuroendocrine phenotype (5). Thyroid transcription factor 1 (TTF-1), a member of the NKX homeobox transcription factor family, is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (6-8). Enolase-2 is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (9). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma and SCLC (10,11). Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is expressed in the secretory vesicles of neurons and endocrine cells (1,2). CHGA is also useful as a serological and immunohistological marker for the presence of neuroendocrine tumors from various tissue sources (12,13). POU2F3 and YAP drive non-neuroendocrine phenotypes. POU2F3 is normally selectively expressed in chemosensory tuft cells, and SCLC expressing POU2F3 resemble that cell type (14). YAP is widely recognized as a key mediator of the Hippo growth signaling pathway (15). Expression of these key biomarkers in SCLC are thought to help predict therapeutic treatment (16).
  1. Sung, H. et al. (2021) CA Cancer J Clin 71, 209-249.
  2. Baine, M.K. et al. (2020) J Thorac Oncol 15, 1823-1835.
  3. Rudin, C.M. et al. (2019) Nat Rev Cancer 19, 289-297.
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