SNAP-Tag (F9J8I) Rabbit mAb #83280
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
SNAP-Tag (F9J8I) Rabbit mAb detects recombinant proteins containing the SNAP epitope tag. The antibody recognizes the SNAP-tag sequence fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed SNAP or CLIP-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.
Species Reactivity:
Source / Purification
Background
SNAP-tag is a 20 kDa mutant of the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) that, when “tagged” to the protein-of-interest, can be used to measure the tagged protein’s expression, localization, and interactions with other proteins. AGT reacts specifically and rapidly with ectopically applied benzylguanin derivative substrates, resulting in covalent labeling of SNAP-tag (1). The benzylguanin derivatives, when labeled with fluorophores, for example, can be used as irreversible probes for SNAP-tagged fusion proteins. SNAP-tag can also be independently detected and labeled using a SNAP-tag protein-specific antibody using a variety of immuno-based assays.
Limited Uses
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