Render Target: STATIC
Render Timestamp: 2024-12-02T11:08:50.095Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:55:12.661
Product last modified at: 2024-09-30T08:01:47.350Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SNF2H (D4W6N) Rabbit mAb #38410

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 125
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SNF2H (D4W6N) Rabbit mAb recognizes endogenous levels of total SNF2H protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H protein.

    Background

    Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (2). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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