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96443
PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet

PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet #96443

Western Blotting Image 1

Western blot analysis of extracts from NCCIT cells either untreated (-) or treated with lambda phosphatase (+) using Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

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Flow Cytometry Image 3

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.

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IF-IC Image 4

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb 92186 100 µl
  • WB
  • IP
H M 35 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb 3579 100 µl
  • WB
  • IF
  • F
H 35 Rabbit 

Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).

Phosphorylation on these and other sites on Sox2 have been observed in pluripotent cells as they undergo differentiation, although the mechanism and consequence of this potential regulation is not clear (10).

  1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
  5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
  6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Okita, K. et al. (2007) Nature 448, 313-7.
  9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.
  10. Van Hoof, D. et al. (2009) Cell Stem Cell 5, 214-26.
Entrez-Gene Id
6657
Swiss-Prot Acc.
P48431
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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